Sains Malaysiana 52(6)(2023): 1649-1670

http://doi.org/10.17576/jsm-2023-5206-04

 

 

Persicaria minor F-box Gene PmF-box1 Indirectly Affects Arabidopsis thaliana LOX-HPL Pathway for Green Leaf Volatile Production

(Gene F-box Persicaria minor PmF-box1 Secara Tidak Langsung Mempengaruhi Tapak Jalan LOX-HPL Arabidopsis thaliana untuk Penghasilan Sebatian Meruap Daun Hijau)

 

NUR-ATHIRAH ABD-HAMID1, MUHAMMAD NAEEM-UL-HASSAN2,3, ZAMRI ZAINAL1,2& ISMANIZAN ISMAIL1,2*

 

1Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

2Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

3Department of Chemistry, University of Sargodha, Sargodha, Punjab 40100, Pakistan

 

Received: 3 October 2022/Accepted: 6 June 2023

 

Abstract

Green leaf volatiles (GLVs) play an essential role in plant defence, plant-plant interaction and plant-insect interaction. The plant releases GLVs and inhibits the growth and propagation of plant pathogens. In this study, overexpression of PmF-box1 in wild type A. thaliana showed the downregulation of genes involved in the lipoxygenase-hydroperoxide lyase (LOX-HPL) pathway, which contributes to the biosynthesis of GLVs. It resulted in a marked reduction of hexanal production in the PmF-box1-overexpressing plant. The expression pattern of LOX-HPL branch genes in the kelch-repeat modified PmF-box1 (KMF)-overexpressing plant showed a pattern much closer to the expression of LOX-HPL branch genes in the vector control (VC) plant. It was shown that the functional KMF protein sequence was not responsible for the significant reduction of all GLVs including hexanal, 1-hexanol, (Z)-3-hexen-1-ol, and the carbon 5 (C5) volatile, 1-penten-3-ol, in plants overexpressing KMF. Furthermore, this study also showed that the relative proportion of production of 1-penten-3-ol to hexanal was higher in the PmF-box1-overexpressing plant. Based on the current comparative literature search, PmF-box1 does not appear to interact directly with the proteins or transcription factors of the LOX-HPL pathway. On the other hand, PmF-box1 interacts with SAMS1, which subsequently influences the HPL pathway enzyme genes. Thus, this study highlights the potential roles of PmF-box1 in the manipulation of GLV productions.

 

Keywords: F-box proteins; hydroperoxide lyase; Kelch-repeats; lipoxygenase; oxylipin

 

Abstrak

Sebatian meruap daun hijau (GLV) memainkan peranan penting dalam pertahanan tumbuhan, interaksi tumbuhan-tumbuhan dan interaksi tumbuhan-serangga. Tumbuhan membebaskan GLV serta merencat pertumbuhan dan propagasi patogen tumbuhan. Dalam kajian ini, pengekspresan lampau PmF-box1 dalam A. thaliana jenis liar telah menunjukkan pengawalaturan menurun gen yang terlibat dalam tapak jalan lipoksigenase-hidroperoksid liase (LOX-HPL) yang menyumbang kepada biosintesis GLV. Ia mengakibatkan pengurangan penghasilan heksanal yang ketara dalam tumbuhan yang mengekspres PmF-box1 secara melampau. Corak pengekspresan gen cabang LOX-HPL dalam tumbuhan yang mengekspres PmF-box1 secara melampau dengan ulangan Kelch (KMF) yang terubah suai menunjukkan corak pengekspresan yang hampir sama dengan gen cabang LOX-HPL di dalam tumbuhan kawalan vektor (VC). Ini menunjukkan bahawa jujukan protein KMF yang berfungsi tidak bertanggungjawab terhadap penurunan yang signifikan bagi semua GLV termasuk heksanal, 1-heksanol, (Z)-3-hexen-1-ol dan karbon 5 (C5) meruap, 1-penten-3-ol, di dalam tumbuhan yang mengekspreskan KMF secara melampau. Tambahan pula, kajian ini juga menunjukkan bahawa perkadaran relatif penghasilan 1-penten-3-ol kepada heksanal adalah lebih tinggi di dalam tumbuhan yang mengekspres PmF-box1 secara melampau. Berdasarkan carian perbandingan kepustakaan semasa, PmF-box1 didapati tidak dapat berinteraksi secara langsung dengan protein atau faktor transkripsi bagi tapak jalan LOX-HPL. Sebaliknya, PmF-box1 berinteraksi dengan SAMS1, yang kemudiannya mempengaruhi gen enzim tapak jalan HPL. Oleh itu, kajian ini menunjukkan PmF-box1 berpotensi berperanan dalam memanipulasi penghasilan GLV.

 

Kata kunci: Hidroperoksid liase; lipoksigenase; oksilipin; protein F-box; ulangan Kelch

 

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*Corresponding author; email: maniz@ukm.edu.my

 

 

 

 

 

 

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