Sains Malaysiana 50(10)(2021): 2923-2936

http://doi.org/10.17576/jsm-2021-5010-07

Essential Oil and Leaves from Lantana camara Significantly Ameliorate Different Cancer Cell Lines by Suppressing the NF-κB Pathway

(Minyak Pati dan Daun daripada Lantana camara secara Signifikan Memperbaiki Garis Sel Kanser Berbeza dengan Merencat Laluan NF-κB)

ARFAA SAJID, QAISAR MANZOOR, ANAM SAJID, RABIA SHABIR AHMAD, GHULAM HUSSAIN, MUHAMMAD IMRAN, FARHAN ASLAM, MUHAMMAD UMAIR ARSHAD, TANWEER ASLAM GONDAL & ALI IMRAN*
 

¹Department of Chemistry, The University of Lahore, Lahore, Pakistan

²Department of Chemistry, University of Punjab, Lahore, Pakistan

³University Institute of Diet and Nutritional Sciences, Faculty of Allied Health Sciences, The University of Lahore-Lahore, Pakistan

⁴Department of Food Science and Human Nutrition, University of Veterinary and Animal Sciences, Lahore, Pakistan

⁵School of Exercise and Nutrition, Deakin University, Victoria 3221, Australia

⁶Institute of Home & Food Sciences, Government College University, Faisalabad, Pakistan

⁷Neurochemicalbiology and Genetics Laboratory (NGL), Department of Physiology, Faculty of Life Sciences, Government College University, Faisalabad, 38000, Pakistan

Diserahkan: 18 Jun 2020/Diterima: 15 Februari 2021

ABSTRACT

The anticancer and anti-inflammatory activities of wild-sage (Lantana camara) leaves essential oil were evaluated against various human cell lines. In this regard, steam hydro-distillation technique was used for oil extraction. Chemical characterization of L. camara essential oil (LCEO) was done by GC-MS, and forty one chemical components (98.69%) were detected in leaves of LCEO. The major constituents were caryophyllene oxide (19.63%), caryophyllene (6.68%), β-phellandrene (6.48%), humulene epoxide II (5.68%), spathulenol (4.17%), α-pinene (3.27%), eucalyptol (3.09%), α-cyclocitral (2.89%), β-pinene (2.38%), and nerolidol II (2.33%). Moreover, the antioxidant and antimicrobial potential of LCEO were also examined. LCEO also showed good anti-inflammatory activity. Interestingly, it was found that the L. camara essential oil was active against a set of microbial strains along with promising antioxidant activity. The MTT assay showed that LCEO possessed good anticancer potential against U-266, A-549, HCT-116, SCC-4, MiaPaCa 2, and KBM-5 cancer cell lines. In these cell lines, it was depicted that the LCEO blocked the tumor cell proliferation via NF-κB pathway suppression. The promising and potent bioactivities of LCEO support the candidature of these essential oils as an anticancer agent.

Keywords: Anti- inflammatory; GC-MS; Lantana camara; MTT assay

ABSTRAK

Aktiviti antikanser dan anti-radang minyak pati daun liar (Lantana camara) dinilai berdasarkan pelbagai titisan sel manusia. Dalam hal ini, teknik penyulingan hidro wap digunakan untuk pengekstrakan minyak. Pencirian kimia minyak pati L. camara (LCEO) dijalankan melalui GC-MS dan empat puluh satu komponen kimia (98.69%) dikesan pada daun LCEO. Konstituen utama adalah kariofilin oksida (19.63%), kariofilin (6.68%), β-fellandren (6.48%), humulena epoksida II (5.68%), spathulenol (4.17%), α-pinene (3.27%), eucalyptol (3.09) %), α-siklokitral (2,89%), β-pinena (2,38%) dan nerolidol II (2,33%). Selain itu, potensi antioksidan dan antimikrob LCEO juga dikaji. LCEO juga menunjukkan aktiviti anti-radang yang baik. Menariknya, didapati bahawa minyak pati L. camara aktif melawan sekumpulan strain mikrob dengan potensi aktiviti antioksidan. Ujian MTT menunjukkan bahawa LCEO memiliki potensi antikanser yang baik terhadap barisan sel barah U-266, A-549, HCT-116, SCC-4, MiaPaCa 2 dan KBM-5. Dalam garis sel ini, digambarkan bahawa LCEO menyekat percambahan sel tumor melalui penekanan laluan NF-κB. Bioaktiviti LCEO yang menjanjikan dan kuat menyokong pencalonan minyak pati ini sebagai agen antikanser.

Kata kunci: Anti-radang; GC-MS; Lantana camara; Ujian MTT

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