Sains Malaysiana 51(11)(2022): 3703-3714

http://doi.org/10.17576/jsm-2022-5111-15

 

Angiotensin-I-Converting Enzyme Inhibitory (ACE-I) Peptide from Germinated Lamtoro Gung (Leucaena leucocephala ssp. glabrata (Rose) S. Zarate) Flour

(Angiotensin-I-Converting Enzyme Inhibitory (ACE-I) Peptida daripada Percambahan Tepung Lamtoro Gung (Leucaena leucocephala ssp. glabrata (Rose) S. Zarate))

 

APRILIA FITRIANI^1,2, RETNO INDRATI¹, YUSTINUS MARSONO¹& SUPRIYADI SUPRIYADI^1,*

 

¹Department of Food Technology and Agricultural Products, Faculty of Agricultural, Technology, Universitas Gadjah Mada, Flora Street 1, Depok, Sleman, Special District of Yogyakarta, 55281, Indonesia

²Food Technology, Faculty of Industrial Technology, Universitas Ahmad Dahlan, Jenderal Ahmad Yani Street, Banguntapan, Bantul, Special District of Yogyakarta, Indonesia

 

Diserahkan: 17 Disember 2021/Diterima: 18 Julai 2022

 

Abstract

Hypertension is a significant public health problem worldwide, a leading risk factor for cardiovascular disease and cause of premature death. Angiotensin-I-converting enzyme (ACE) activity is one of the causes of hypertension. Lamtoro Gung has potential as an Angiotensin-I-converting Enzyme Inhibitory (ACE-I) due to the presence of peptide that able to inhibit its activity to prevent hypertension. The germination process was carried out to hydrolyse storage proteins and produce peptides that have a low molecular weight. This study investigated ACE-I activity from Lamtoro Gung seed during germination and evaluated the blanching effect on it. This experiment was conducted with a Completely Randomised Design (CRD), and the factor is the differences in germination duration (0, 12, 24, 36, 48, 60, and 72 h). Proteolytic activity and the degree of hydrolysis during germination were studied to know the correlation between germination and ACE-I activity. The highest ACE-I activity sample was blanched with three different durations (2, 4, and 6 s). The 48 h germinated Lamtoro Gung had the highest ACE-I activity (70.62%). This result was supported by the proteolytic activity (168.79 U/g protein dry matter) and degree of hydrolysis (23.26%). Forty-eight hours of germination of Lamtoro Gung resulted in the highest ACE-I activity. Blanching of germinated Lamtoro Gung for 2 s could hold the ACE-I activity, but the longer duration decreased it.

 

Keywords: ACE-I; blanching; germination; Lamtoro Gung

 

Abstrak

Hipertensi adalah masalah kesihatan awam utama di seluruh dunia, faktor risiko utama penyakit kardiovaskular dan punca kematian pramatang. Aktiviti Angiotensin-I-converting Enzyme (ACE) adalah salah satu punca hipertensi. Lamtoro Gung berpotensi sebagai Angiotensin-I-converting Enzyme Inhibitory (ACE-I) kerana kehadiran peptida yang mampu menghambat aktivitinya untuk mencegah hipertensi. Proses percambahan boleh dijalankan untuk menghidrolisis protein simpanan dan menghasilkan peptida ringkas. Penyelidikan ini mengkaji aktiviti ACE-I daripada biji Lamtoro Gung semasa percambahan dan menilai kesan kukus kepadanya. Uji kaji ini dijalankan dengan Reka Bentuk Rawak Sepenuhnya (CRD) dan faktor penelitian yang digunakan iaitu perbezaan dalam tempoh percambahan (0, 12, 24, 36, 48, 60 dan 72 jam). Aktiviti proteolitik dan tahap hidrolisis semasa percambahan dikaji untuk mengetahui perkaitan antara percambahan dan aktiviti ACE-I. Sampel aktiviti ACE-I tertinggi kemudian dikukus dengan tiga tempoh didih yang berbeza (2, 4 dan 6 s). Lamtoro Gung yang bercambah 48 jam mempunyai aktiviti ACE-I tertinggi (70.62%). Keputusan ini disokong oleh aktiviti proteolitik (168.79 U/g protein bahan kering) dan tahap hidrolisis (23.26%). Lamtoro Gung yang bercambah dan dikukus selama 2 s boleh menahan aktiviti ACE-I, tetapi tempoh yang lebih lama mengurangkannya.

 

Kata kunci: ACE-I; Lamtoro Gung; pengukusan; percambahan

 

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