Sains Malaysiana
49(3)(2020): 545-552
http://dx.doi.org/10.17576/jsm-2020-4903-09
Production and Characterization
of Spray-Dried Swamp Eel (Monopterus
albus) Protein Hydrolysate
Prepared by Papain
(Pengeluaran dan
Pencirian Hidrolisat Protein Belut Paya
Semburan Kering (Monopterus albus) disediakan melalui
Papain)
SRI PRIATNI1*, KEZIA HARIMADI2, EFENDI BUANA2, WAWAN KOSASIH1
& ROHMATUSSOLIHAT ROHMATUSSOLIHAT3
1Research Unit for Clean Technology, Indonesian Institute of Sciences, Indonesia
2Surya University, Indonesia
3Research Center for Biotechnology LIPI, Indonesia
Diserahkan: 2 September 2019/Diterima:
5 Disember 2019
ABSTRACT
Protein
hydrolysate from swamp eel (Monopterus albus) has been prepared by enzymatic hydrolysis process
using papain enzyme. Evaluation of the extent of protein hydrolysis
was conducted by measuring the degree of hydrolysis (DH). The optimization
of protein hydrolysate production has been carried out by analyzing
the influences of papain enzyme concentration, temperature, and
time of hydrolysis on the degree of hydrolysis (DH) using RSM design.
The optimized product was spray-dried and analyzed the proximate
(moisture, lipid, protein) content and the yield (%). The fish protein
hydrolysate (FPH) powder product was characterized by the foaming
capacity and stability, and also by FTIR, DSC and PSA methods. The
optimum condition of enzymatic hydrolysis of swamp eel protein was
obtained by an addition 0.49 % of papain enzyme at 45oC
for 9 hours. The degree of hydrolysis (DH) of this product was 7.96
% with a yield of 14.72 %. The foaming capacity of swamp eel protein
hydrolysate powder was between 12.5 - 62.5 % and the foaming stability
was between 3.22 - 31.25 %. The highest foaming capacity and stability
of this product was reached at pH 4.0. Based on the spectrum FTIR
analysis, the FPH product contained amines, aromatics, aliphatics,
amide B and amide II groups. DSC analysis of the FPH product showed
two peaks (Tm) at 65 oC and
108.5 oC. The particle size
of the FPH powder product was distributed within 100 nm-1500 nm range with
the highest intensity was 8.91 %. This study shows the potential
usage of swamp eel for the production of FPH by enzymatic hydrolysis
using papain enzyme with high yield and serves as a protein supplement.
Keywords: Monopterus albus; papain; protein hydrolysate
Abstrak
Hidrolisat protein daripada belut paya (Monopterus albus) telah disediakan melalui proses hidrolisis enzim dengan menggunakan enzim papain. Penilaian tahap hidrolisis
protein telah dijalankan
dengan mengukur darjah hidrolisis (DH). Pengoptimuman pengeluaran hidrolisat protein telah dijalankan dengan menganalisis kepekatan enzim papain, suhu dan masa hidrolisis
pada darjah
hidrolisis (DH) menggunakan reka bentuk RSM. Produk yang dioptimumkan adalah sembur-kering dan analisis kandungan proksimat serta hasil (%). Produk serbuk protein hidrolisat
(FPH) dicirikan melalui
kapasiti buih dan
kestabilan serta
kaedah FTIR, DSC dan PSA.
Keadaan yang optimum
untuk enzim
hidrolisis daripada protein belut paya diperoleh
dengan penambahan
0.49 % enzim papain pada 45 oC untuk 9 jam.
Darjah
hidrolisis (DH) produk ini adalah 7.96 % dengan hasil sebanyak
14.72 %. Kapasiti buih serbuk hidrolisat protein belut paya adalah antara
12.5-62.5 % dan kestabilan
buih adalah antara
3.22-31.25 %. Kapasiti buih tertinggi
dan kestabilan
produk ini telah
dicapai pada
pH4.0. Berdasarkan analisis spektrum FTIR, produk FPH mengandungi kumpulan amina, aromatik, alifatik, amida B dan amida
II. Analisis DSC produk FPH menunjukkan
dua puncak (Tm)
pada 65 oC dan 108.5 oC.
Saiz zarah produk serbuk
FPH diagihkan dalam
julat lingkungan 100 nm-1500 nm
dengan keamatan tertinggi adalah pada 8.91 %. Kajian ini menunjukkan
potensi penggunaan
belut paya untuk
pengeluaran FPH melalui
hidrolisis enzim menggunakan enzim papain dengan hasil yang tinggi dan berfungsi
sebagai protein tambahan.
Kata
kunci: Hidrolisat
protein; Monopterus albus; papain
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*Pengarang untuk surat-menyurat; email: sripriatni@gmail.com
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