Sains Malaysiana 47(12)(2018): 3051–3059

http://dx.doi.org/10.17576/jsm-2018-4712-15

 

Sesquiterpene Production in Methyl Jasmonate-Induced Persicaria minor Cell Suspension Culture

(Penghasilan Seskuiterpena dalam Kultur Ampaian Sel Persicaria minor Diinduksi Metil Jasmonat)

 

PIRIYATASNI SELLAPAN, EMELDA ROSSELEENA ROHANI & NORMAH MOHD NOOR*

 

Institute of Systems Biology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

 

Diserahkan: 30 Mei 2018/Diterima: 18 September 2018

 

ABSTRACT

Sesquiterpenes are a three-isoprene unit compounds which belong to terpenoid family of secondary metabolites. These volatile compounds are one of the major constituents of essential oils in plants and plays major roles in plant signaling of defense mechanism. The effects of methyl jasmonate (MeJA) concentrations (100 and 200 μM) on the production of sesquiterpene compounds after incubation period for 1, 3, and 6 days were investigated in Persicaria minor cell suspension culture. Headspace Solid-Phase Microextraction (HS-SPME) method was used to absorb volatile compounds from suspension cells and liquid medium. They were then analyzed through Gas Chromatography-Mass Spectrometry (GC-MS) to identify sesquiterpene compounds. Among the 15 sesquiterpene compounds identified, α- muurolene was found in significantly higher concentration in all MeJA treated cultures. The results showed that α-muurolene was detected in the suspension cells at the highest peak area of 14.17% at 100 μM MeJA treated cultures with 3-day incubation. Analysis of liquid medium of the treatments identified secretion of α-muurolene into the culture medium, with total peak area of 0.72%. These results showed that sesquiterpene production in MeJA induced P. minor suspension culture depended on the MeJA concentration and also culture incubation period.

 

Keywords: α-muurolene; cell suspension culture; GC-MS; methyl jasmonate; sesquiterpene

 

ABSTRAK

Seskuiterpena adalah unit sebatian tiga isoprena yang tergolong dalam keluarga terpenoid metabolit sekunder. Sebatian meruap ini adalah juzuk utama dalam minyak pati daripada tumbuhan dan memainkan peranan penting sebagai pengisyaratan bagi mekanisme pertahanan tumbuhan. Kesan kepekatan metil jasmonat (MeJA) (100 dan 200 μM) ke atas penghasilan seskuiterpena dengan tempoh masa pengeraman 1, 3 dan 6 hari telah dikaji dalam kultur ampaian sel Persicaria minor. Pengekstrakan-mikro ruang kepala fasa (Headspace Solid-Phase Microextraction) (HS-SPME) bersama kromatografi gas-spektrometri jisim (GC-MS) digunakan untuk mengenal pasti sebatian seskuiterpena daripada ekstrak ampaian sel. Daripada 15 sebatian seskuiterpena yang dikenal pasti, α-muurolin didapati hadir pada kepekatan yang signifikan bagi kesemua kultur yang diberi perlakuan MeJA. Keputusan kajian menunjukkan α-muurolin dikesan pada luas puncak tertinggi 14.71% dalam ampaian sel pada 100 μM MeJA dengan tiga hari pengeraman. Analisis ke atas medium cecair mendapati kehadiran rembesan α-muurolin dengan luas puncak keseluruhan 0.72%. Keputusan menunjukkan bahawa penghasilan seskuiterpena dalam ampaian sel terinduksi MeJA bagi P. minor adalah bergantung kepada kepekatan MeJA dan juga tempoh pengeraman.

 

Kata kunci: α-muurolin; GC-MS; kultur ampaian sel; metil jasmonat; seskuiterpena

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*Pengarang untuk surat-menyurat; email: profnormah@yahoo.com