Sains Malaysiana 46(9)(2017): 1401–1405

http://dx.doi.org/10.17576/jsm-2017-4609-07

Triclopyr 3, 5, 6-Trichloro-2-Pyridinyl Clean-Up Procedure from Soil, Sediment and Water Samples Using SPE-HPLC-VWD

(Tatacara Pembersihan Triklopir 3, 5, 6-Trikloro-2-Piridinil daripada Sampel Tanah,

Sedimen dan Air Menggunakan SPE-HPLC-VWD)

TAYEB, M.A.¹*, ISMAIL, B.S. ¹, MARDIANA-JANSAR, K.¹, GOH CHOO TA² & AFROZA, Y.M.²

¹School of Environmental and Natural Resource Sciences, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

²Institute for Environment and Development (LESTARI), Universiti Kebangsaan Malaysia

43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

Diserahkan: 31 Ogos 2016/Diterima: 18 April 2017

ABSTRACT

Triclopyr is a post emergence herbicide used to control woody plants. After application, the excess amount will enter the soil and water bodies and it is present in ppb level thus making extraction very difficult. The extraction of triclopyr 3, 5, 6-trichloro-2-pyridinyl residue from soil, sediment and water samples under different solid phase extraction (SPE) sorbent efficiency was studied for better recovery. Four different SPE sorbents i.e.: Oasis HLB, Water Sep-Pak, Cromabond (cation/anion PS-H⁺ /OH-), Isolute ENV⁺ and a series of solvent i.e. potassium dihydrogen phosphate (KH₂PO₄ 0.1M), sodium hydroxide (NaOH 0.2M), potassium hydroxide (KOH 0.5 & 0.6M), ammonium acetate, methanol and water were used as extraction solution. Sample clean-up performance was evaluated using high performance liquid chromatography (HPLC, Agilent 1220 infinity LC) with variable wavelength detector (VWD) 290 nm. Cromabond®H+/OHcolumn with 0.6 M KOH was the most suitable for the clean-up in view of the overall feasibility of the analysis. The highest recovery was 89.32%.

Keywords: Clean-up; sorbents; triclopyr; water samples

ABSTRAK

Triklopir adalah herbisid selepas percambahan (herbisid sistemik) yang digunakan untuk pengawalan tumbuhan berkayu. Selepas penyemburan, lebihan herbisid akan masuk ke dalam tanah dan badan air dan hadir pada aras ppb yang menjadikannya sukar untuk diekstrak. Pengekstrakan triklopir 3,5,6-trikloro-2-piridinil daripada sampel tanah, sedimen dan air dengan menggunakan kecekapan penjerapan pengekstrakan fasa pepejal (SPE) berbeza telah dikaji untuk perolehan yang lebih baik. Empat penjerap SPE berbeza iaitu Oasis HLB, Water Sep-Pak, Cromaibond (kation/anion PS-H+ /OH-), Isolute ENV+ dan beberapa siri pelarut seperti kalium dihidrogen fosfat (KH2PO4 0.1M), natrium hidroksida (NaOH 0.2M), kalium hidroksida (KOH 0.5 & 0.6M), ammonium asetat, metanol dan air telah digunakan sebagai larutan pengekstrakan. Prestasi pembersihan dinilai menggunakan kromatografi cecair berprestasi tinggi (HPLC), (Agilent 1220 infiniti LC) dengan pengesan panjang gelombang pemboleh ubah (VWD) 290 nm. Turus Cromabond®H+/OH dengan 0.6 M KOH adalah yang paling sesuai bagi kaedah pembersihan dengan melihat keseluruhan analisis. Perolehan tertinggi bagi triklopir adalah 89.32%.

Kata kunci: Pembersihan; penjerap; sampel air; triklopir

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*Pengarang untuk surat-menyurat; email: atayeb91@yahoo.com