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Sains Malaysiana 46(8)(2017):
1249–1257 http://dx.doi.org/10.17576/jsm-2017-4608-10
Efficiency of Developed
Solid State Bioreactor 'FERMSOSTAT' on Cellulolytic and Xylanase
Enzymes Production (Kecekapan Bioreaktor
Keadaan Pepejal ‘FERMSOSTAT’ yang Dibangunkan
C.K. LEE1,2*, DARAH, I.1 & IBRAHIM, C.O.3
1Industrial
Biotechnology Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, 11800
USM, Penang, Pulau Pinang, Malaysia
2Bioprocess
Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800 USM, Penang, Pulau Pinang, Malaysia
3Universiti
Malaysia Kelantan, Campus Jeli, Beg Berkunci No 100, 17600 Jeli,
Kelantan Darul Naim, Malaysia
Diserahkan: 22 Januari 2016/Diterima: 1 Februari 2017
ABSTRACT
FERMSOSTAT is a developed laboratory scale solid state fermenter. It is a horizontal
stirrer drum bioreactor with about 70 L capacities. The fermenter
is made of stainless steel which is anti-corrosive and non-toxic
to the process organism. The fermenter is equipped with sets of
control systems for temperature, agitation, aeration and also outlets
for substrate sampling as well as inlets for inoculation and substrate
additions. The uniqueness of this FERMSOSTAT
system is its ability to carry out in situ substrate
sterilization and extraction of enzymes at the end of SSF process. Moreover, the mixing
system provided by FERMSOSTAT can be performed either full
or half mixing as well as forward or reverse mixing. Furthermore,
the mixing can be programmed to run at certain agitation rate and
time interval during the fermentation process to prevent or reduce
damage to the fungus mycelia. FERMSOSTAT is a developed SSF bioreactor
and not an improvement of any existing one. The performances of
FERMSOSTAT have
been evaluated. Under optimum solid state fermentation conditions,
about 63.4, 397 and 3.21 U/g of CMCase, xylanase and FPase activities were detected, which were higher
compared to the tray system.
Keywords: Cellulase; palm kernel cake; solid state fermentation;
sugarcane bagasse; xylanase
ABSTRAK
FERMOSTAT
adalah fermenter keadaan pepejal
berskala makmal
yang dibangunkan. Ia adalah
bioreaktor pengacau
gendang mendatar dengan kapasiti kira-kira 70 L. Fermenter ini
terdiri daripada keluli tahan karat yang anti
hakis dan tidak
toksik terhadap
organisma. Fermenter ini
dilengkapi dengan
sistem kawalan suhu, penggoncangan, pengudaraan dan juga salur keluar untuk
persampelan substrat
dan salur masuk
untuk inokulasi
dan penambahan substrat. Keunikan sistem FERMOSTAT ini
adalah keupayaan
untuk menjalankan pensterilan substrat secara in situ dan pengekstrakan enzim pada akhir proses SSF.
Selain itu, sistem
pencampuran yang dilengkapi
oleh FERMSOSTAT boleh
melakukan pencampuran
secara penuh atau
separuh penuh
dan juga pencampuran ke depan atau
sebaliknya. Tambahan
pula, pencampuran boleh diprogramkan untuk melakukan kadar
pergolakan yang berlainan
dan pada selang
masa yang berbeza sepanjang
masa proses penapaian untuk
mengelak atau
mengurangkan kecederaan pada miselium kulat. FERMSOSTAT adalah SSF
bioreaktor yang telah dibangunkan dan bukan penambahbaikan daripada yang sedia ada. Prestasi FERMSOSTAT telah dinilai. Dalam keadaan optimum fermentasi keadaan pepejal, kira-kira 63.4, 397 dan 3.21
U/g aktiviti CMCase,
xilanase dan
FPase telah dikesan
dengan nilainya
adalah lebih tinggi
berbanding dengan
sistem dulang. Kata kunci: Fermentasi keadaan pepejal; hampas tebu; isirong
kelapa sawit;
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*Pengarang untuk surat-menyurat;
email: cklee1311@yahoo.co.uk
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