Sains Malaysiana 45(4)(2016): 559–565

Bioactivity and Stability Studies of Anthocyanin-Containing Extracts from Garcinia mangostana L. and Etlingera elatior Jack

(Aktiviti Biologi dan Kajian Kestabilan Ekstrak mengandungi Antosianin daripada

Garcinia mangostana L. dan Etlingera elatior Jack)

SUJATHA RAMASAMY*, NOR AFIFA MAZLAN, NURUL ASYIKIN RAMLI, WAN NUR ASYIQIN RASIDI & SUGUMARAN MANICKAM

Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia

Diserahkan: 11 Mac 2015/Diterima: 9 Oktober 2015

ABSTRACT

Anthocyanin-containing extract (ACE) were prepared from the pericarp of Garcinia mangostana L. (mangosteen) and the inflorescence part of Etlingera elatior Jack (torch ginger) by using acidified methanol as extraction solvent. Our present study focuses on the antioxidant and cytotoxic activity and the effect of temperature, light and pH on stability of ACE derived from G. mangostana and E. elatior. The monomeric anthocyanin and total phenolic content in E. elatior was higher (43.42±0.01 mg/L dan 10.07±0.01 gGAE /100 g) compared to G. mangostana (15.03±0.01 mg/L and 3.29±0.01 gGAE/100 g). Both ACE of E. elatior and G. mangostana exhibited free radical scavenger activity, with IC₅₀ value of14.90±1.02 mg/mL and 15.50±0.52 mg/mL, respectively, in 2,2’-diphenyl-1-picrylhydrazyl assay. ACE of E. elatior was also found to be a good reducing and possessed higher absorbance values at concentrations range from 1.25 to 20 mg/mL in FRAP assay. ACE of  E. elatior exhibited mild cytotoxicity on human ovarian SKOV-3 cell line with IC₅₀ values of 54.32 ± 4.60 μg/mL. The effects of light and temperature on ACE stability were performed in different environmental conditions, which promote the destabilization of anthocyanin molecules. ACE stability of G. mangostana were less resist to the effect of light but very susceptible to the prolonged effect of heat after 2 h exposure compared to the ACE in E. elatior. The different in pH highly influence the stability of both ACE which can be observed from the decrease in absorbance readings.

Keywords: Antioxidant; anthocyanin; cytotoxic; Etlingera elatior Jack; Garcinia mangostana L.

ABSTRAK

Antosianin yang mengandungi ekstrak (ACE) telah disediakan daripada perikarp Garcinia mangostana L. (Manggis) dan bahagian bunga Etlingera elatior Jack (kantan) dengan menggunakan metanol berasid sebagai pelarut pengekstrakan. Kajian ini memberi tumpuan kepada aktiviti antioksida dan sitotoksik dan kesan suhu, cahaya dan pH pada kestabilan ACE daripada G. mangostana dan E. elatior. Kandungan antosianin monomer dan jumlah kandungan fenolik dalam E. elatior adalah lebih tinggi (43.42±0.01 mg/L dan 10.07±0.01 gGAE /100 g) berbanding dengan G. mangostana (15.03±0.01 mg/L dan 3.29±0.01 gGAE/100 g). Kedua-dua ACE E. elatior dan G. mangostana menunjukkan aktiviti pemakan radikal bebas dengan nilai IC₅₀ sebanyak 14.90±1.02 mg/mL dan 15.50± 0.52 mg/mL, masing-masing dalam asei 2,2’-difenil-1-picrylhydrazyl. ACE E. elatior juga didapati mempunyai penurunan yang baik dan nilai absorbans yang tinggi pada kepekatan antara 1.25 hingga 20 mg/L dalam asei FRAP. ACE E. elatior menunjukkan sitotoksisiti sederhana pada leluhur sel kanser SKOV-3 ovari dengan nilai IC₅₀ 54.32 ± 4.6 0 μg/mL. Kesan cahaya dan suhu terhadap kestabilan ACE telah dijalankan dalam keadaan persekitaran yang berbeza, yang menggalakkan ketidakstabilan molekul antosianin. Kestabilan ACE G. mangostana kurang menahan kesan cahaya tetapi sangat terdedah kepada kesan cahaya yang berpanjangan selama dua jam berbanding ACE E. elatior. Perbezaan dalam pH sangat mempengaruhi kestabilan kedua-dua ACE yang dapat dilihat daripada penurunan dalam bacaan absorbans.

Kata kunci: Antioksida; antosianin; Etlingera elatior Jack; Garcinia mangostana L.; sitotoksik

RUJUKAN

Boranbayeva, T., Karadeniz, F. & Yilmaz, E. 2014. Effects of storage on anthocyanin degradation in black mulberry juice and concentrates. Food Bioprocess Technology 7: 1894-1902.

Cassidy, A., Rogers, G., Peterson, J., Dwyer, J.T., Lin, H. & Jacques, P.F. 2015. Higher dietary anthocyanin and flavonol intakes are associated with anti-inflammatory effect in a population of US adults. The American Journal of Clinical Nutrition 102(1): 172-181.

Chan, E.W.C., Lim, Y.Y. & Omar, M. 2007. Antioxidant and anti-bacterial activity of leaves of Etlingera species (Zingiberaceae) in Peninsular Malaysia. Food Chemistry 104: 1586-1593.

Chattopadhyay, P., Chatterjee, S. & Sen, S.K. 2008. Biotechnological potential of natural food grade biocolorants. African Journal of Biotechnology 7(17): 2972-2985.

Farah, S.H., Wende, L. & Trust, B. 2008. Measurement of anthocyanins and other phytochemicals in purple wheat. Food Chemistry 109: 916-924.

Ge, Q. & Ma, X. 2013. Composition and antioxidant activity of anthocyanins isolated from Yunnan edible rose (An ning). Beijing Academy of Food Science 2: 68-74.

Giusti, M.M. & Wrolstad, R.E. 2001. Characterization and measurement of anthocyanins by UV-visible spectroscopy. In Current Protocols in Food Analytical Chemistry, edited by Wrolstad, R.E. New York: John Wiley & Sons. pp. 1-13.

Hou, Z., Qin, P., Zhang, Y., Cui, S. & Ren, G. 2013. Identification of anthocyanins isolated from black rice (Oryza sativa L.) and their degradation kinetics. Food Research International 50(2): 691-697.

Jaakola, L. 2013. New insights into the regulation of anthocyanin biosynthesis in fruits. Trends in Plant Science 18(9): 477-483.

Junqueira-Goncalves, M.P., Yanez, L., Morales, C., Navarro, M., Contreras, R.A. & Zuniga, G.E. 2015. Isolation and characterization of phenolic compounds and anthocyanins from Murta (Ugni molinae Turcz.) Fruits. Asssessment of Antioxidant and Antibacterial Activity. Molecules 20(4): 5698-5713.

Jusoh, S.A.M., Johan, M.F. & Mohamed, A.S. 2012. Anti-proliferative and pro-apoptotic effect of P. sacharosa, E. elatior and P. granatum aqueous extract on human myeloid leukaemia: A preliminary study. Asian Journal of Medical Research 1(4): 146-151.

Lachumy, S.J.T., Sasidharan, S., Sumathy, V. & Zuraini, Z. 2010. Pharmacological activity, phytochemical analysis and toxicity of methanol extract of Etlingera elatior (torch ginger) flowers. Asian Pacific Journal of Tropical Medicine 3: 769-774.

Liu, J., Jia, L., Kan, J. & Jin, C.H. 2013. In vitro and in vivo antioxidant activity of ethanolic extract of white button mushroom (Agaricus bisporus). Food and Chemical Toxicology 51: 310-316.

Longo, L. & Vasapollo, G. 2006. Extraction and identification of anthocyanins from Smilax aspera L. berries. Food Chemistry 94: 226-231.

Luo, H., Lv, X.D., Wang, G.E., Li, Y.F., Kurihara, H. & He, R.R. 2014. Anti-inflammatory effects of anthocyanins-rich extract from bilberry (Vaccinium myrtillus L.) on croton oil-induced ear edema and Propionibacterium acnes plus LPS-induced liver damage in mice. International Journal of Food Sciences and Nutrition 65(5): 594-601.

Marco, P.H., Poppi, R.J., Scarminio, I.S. & Tauler, R. 2011. Investigation of the pH effect and UV radiation on kinetic degradation of anthocyanin mixtures extracted from Hibiscus acetosella. Food Chemistry 125(3): 1020-1027.

Peng, S. & Zhao, M. 2009. Pharmaceutical Bioassays: Methods and Applications. New York: Wiley Publications.

Ramasamy, S., Abdul Wahab, N., Zainal Abidin, N., Manickam, S. & Zakaria, Z. 2013. Growth inhibition of human gynecologic and colon cancer cells by Phyllanthus watsonii through apoptosis induction. PLoS ONE 7(4): e34793.

Reque, P.M., Steffens, R.S., Jablonski, A., Flores, S.H., de O Rios, A. & de Jong, E.V. 2014. Cold storage of blueberry (Vaccinium spp.) fruits and juice: Anthocyanin stability andantioxidant activity. Journal of Food Composition and Analysis 33(1): 111-116.

Reshmi, S.K., Aravindhan, K.M. & Suganya Devi. 2012. The effect of light, temperature, pH stability of betacyanin pigments in Basella alba fruit. Asian Journal of Pharmaceutical and Clinical Research 5(4): 107-110.

Rugina, D., Sconta, Z., Leopold, L., Pintea, A., Bunea, A. & Socaciu, C. 2012. Antioxidant activities of chokeberry extracts and the cyotoxic action of their anthocyanin fraction on HeLa human cervical tumor. Journal of Medicinal Food 15(8): 700-706.

Sancho, R.A.S. & Pastore, G.M. 2012. Evaluation of the effects of anthocyanins in type 2 diabetes. Food Research International 46(1): 378-386.

Solyom, K., Sola, R., Cocero, M.J. & Mato, R.B. 2014. Thermal degradation of grape marc polyphenols. Food Chemistry 159: 361-366.

*Pengarang untuk surat-menyurat; email: sujatha@um.edu.my