Sains Malaysiana 43(2)(2014): 233–240

Ko-pengekspresan Reseptor Estrogen beta (ERβ) dan Aktin Otot Licin pada Tumor Filodes

di Payudara: Suatu Kajian Tisu Mikroarai

(The Co-expression of Estrogen Receptor beta (ERβ) and Smooth Muscle Actin in Phyllodes Tumour of Breast: A Tissue Microarray Study)

NURHAYATI H. MUNAWER¹, SITI-AISHAH MD ALI*¹, REENA MD ZIN¹

& ROHAIZAK MUHAMMAD²

¹Jabatan Potologi, Pusat Perubatan Universiti Kebangsaan Malaysia (PPUKM)

56000 Kuala Lumpur, Malaysia

²Jabatan Surgeri, Pusat Perubatan Universiti Kebangsaan Malaysia (PPUKM)

56000 Kuala Lumpur, Malaysia

Diserahkan: 6 Disember 2012/Diterima: 18 Mei 2013

ABSTRAK

Proliferasi tumor filodes tertumpu terutama pada bahagian stroma yang dianggap sebagai komponen neoplastik bagi tumor filodes. Reseptor Estrogen (ER) yang memainkan peranan dalam payudara neoplastik juga terlibat dalam perkembangan tumor filodes. ERβ adalah satu jenis klon ER yang dilaporkan hadir pada stroma tumor payudara manakala pengekspresan aktin otot licin (SMA) di stroma dapat membandingkan gred histologi tumor filodes. Kami membandingkan pengekspresan ERβ dengan SMA pada komponen stroma tumor filodes menggunakan teknik tisu mikroarai (TMA). TMA dibentuk ke atas 77 kes tumor filodes (46 benigna, 17 pinggiran dan 14 malignan) menggunakan jarum berdiameter 0.6 mm (Alphelys Plaisir, Perancis) dan pewarnaan imunohistokimia dijalankan menggunakan penanda molekul ERβ dan SMA. Tumor filodes kerap hadir pada wanita berusia lebih daripada 40 tahun dengan tumor filodes benigna menunjukkan median umur pesakit paling rendah (p=0.017). Ekspresi ERβ dalam komponen stroma meningkat dengan gred histologi tumor. Sementara SMA menunjukkan ekspresi pada 62.8, 41.2 dan 57.1%, masing-masing bagi tumor filodes benigna, pinggiran dan malignan. Kedua-dua ERβ (p=0.024) dan SMA lebih cenderung hadir pada wanita ≥40 tahun. Kajian menunjukkan hubungan signifikan antara ko-pengekspresan ERβ dan SMA (p=0.047) dan 65.5% daripadanya adalah wanita berumur lebih daripada 40 tahun. Ekspresi SMA yang tinggi pada stroma tumor filodes benigna mungkin menunjukkan potensi proliferasi tumor ini untuk menjadi malignan. Ekspresi tinggi ERβ dengan tumor filodes malignan dan hubungannya dengan SMA mencadangkan ko-pengekspresan kedua-dua penanda molekul ini mungkin berperanan dalam tumorigenesis stroma tumor filodes.

Kata kunci: ERβ; SMA; tisu mikroarai; tumor filodes

ABSTRACT

Proliferation of phyllodes tumour is mainly in the stromal that is considered as the neoplastic component of phyllodes tumour. Estrogen Receptor (ER) that plays an important role in breast neoplasm is also involved in progression of phyllodes tumour. ERβ is a type of ER which was reported to be expressed in the breast stroma while the expression of smooth muscle actin (SMA) in the stroma may differentiate histological grade of phyllodes tumour. We compared the expression of ERβ to SMA in stromal component of phyllodes tumour using tissue microarray (TMA) technique. A TMA was constructed on 77 cases of phyllodes tumour (46 benign, 17 boderline and 14 malignant) using 0.6 mm punch kit (Alphelys Plaisir, France) and immunohistochemical staining was done using ERβ and SMA. Phyllodes tumour seen in women aged more than 40 year-old with benign phyllodes tumour showed the lowest median of age (p=0.017). ERβ expression in stromal component increased with histological grade of the tumour. SMA was positive in 62.8, 41.2 and 57.1%, of benign, borderline and malignant phyllodes tumour, respectively. Both ERβ (p=0.024) and SMA were commonly expressed in women aged ≥40 years. We found a significant co-expression of ERβ and SMA (p=0.047) and 65.5% of these cases occured in women aged more than 40 years-old. The high expression of SMA in stroma of benign phyllodes tumour may demonstrate the potential of proliferation of this tumour to become malignant. High expression of ERβ of malignant phyllodes tumour and its significant association with SMA suggests that co-expression of these two markers may play a role in stromal tumorigenesis of phyllodes tumour.

Keywords: ERβ; phyllodes tumour; SMA; tissue microarray

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*Pengarang untuk surat-menyurat; email: saishah@ppukm.ukm.edu.my