Sains Malaysiana 43(2)(2014):
195–201
Penyaringan Aktiviti Biologi
Pelbagai Ekstrak dan Polisakarida Cendawan
Amauroderma sp. dari Taman Negeri Royal
Belum
(Bioactivity Screening of Various Extracts and Polysaccharide of
Local
Mushroom Amauroderma sp. from Royal Belum State Park)
ENDOM ISMAIL1*, FATIMAH DIANA AMIN NORDIN2, FAUZI DAUD1, MAT RASOL AWANG3 & NAZLINA
IBRAHIM1
1School of Bioscience and Biotechnology, Faculty of Science
and Technology
Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
2Clinical Research Centre (CRC), Serdang Hospital, 43000 Kajang,
Selangor, Malaysia
3Agensi
Nuklear Malaysia, 43000 Bangi, Selangor, Malaysia
Diserahkan: 6 Disember 2012/Diterima: 19 Mei 2013
ABSTRAK
Kajian ini bertujuan untuk melakukan penyaringan pelbagai aktiviti
biologi menggunakan ekstrak akues rebusan air, ekstrak metanol larut air,
ekstrak etanol, ekstrak metanol dan polisakarida miselium cendawan Amauroderma sp. yang diperoleh dari Taman
Negeri Royal Belum. Ujian antikanser, antivirus, antibakteria dan antioksida
digunakan dalam penyaringan ini. Ujian antikanser dijalankan ke atas sel kanser
ovari (CaOV-3) dan sel normal hati Chang dengan tamoxifen digunakan sebagai
kawalan. Kesemua nilai IC50 yang diperoleh menunjukkan nilai yang tinggi
(400±3.6 - 3950±0.005 μg/mL). Sementara itu, kajian awal penyaringan
aktiviti antivirus dimulakan dengan ujian sitotoksisiti ekstrak terhadap sel
Vero. Didapati hanya ekstrak rebusan air dan etanol memberi nilai CC50 masing-masing
pada kepekatan 6.4±0.3 dan 7.9±1.28 mg/mL. Seterusnya, ujian antivirus
menggunakan virus Herpes Simpleks Jenis 1 (HSV-1) yang menjangkiti
sel Vero dilakukan dengan dua kaedah iaitu pra-rawat dan pos-rawat. Ekstrak
menunjukkan aktiviti yang tidak ketara terhadap HSV-1 bagi kedua-dua
kaedah ini. Penyaringan aktiviti antibakteria dilakukan ke atas beberapa jenis
bakteria gram negatif dan positif dengan satu siri kepekatan ekstrak; 10, 5,
2.5, 1.25 dan 0.625 mg/mL. Kesemua ekstrak tidak menunjukkan aktiviti
antibakteria berdasarkan ketiadaan sebarang zon perencatan dalam kesemua piring
petri. Walau bagaimanapun, penyaringan antioksida menunjukkan kadar peratusan
penyingkiran radikal bebas yang tinggi terutamanya ekstrak etanol iaitu 93.6%.
Ini diikuti oleh ekstrak rebusan air (92.65%), metanol (88.83%) serta
polisakarida (85.75%). Memandangkan kesemua ekstrak tidak menunjukkan kehadiran
aktiviti sitotoksik, antivirus dan antibakteria, tetapi mempunyai aktiviti
antioksida yang signifikan, maka bolehlah disimpulkan bahawa pelbagai ekstrak Amauroderma
sp. sangat berpotensi secara khusus sebagai komponen nutrisi bersifat
antioksidatif.
Kata kunci: Amauroderma sp.; antibakteria; antioksida; antivirus; sitotoksisiti
ABSTRACT
This study aimed to screen for various biological activities using
aqueous extract, methanol: water extract, ethanol extract, methanol extract and
polysaccharide from mycelium of Amauroderma sp. obtained from Royal Belum State Park.
Anticancer, antivirus, antibacterial and antioxidant tests were used in this
screening. Anticancer test were done using human ovarian cell line (CaOV-3) and
Chang’s normal liver cells with tamoxifen as a control. All IC50 values obtained in this
test is high (400±3.6 - 3950±0.005 μg/ml). Meanwhile, early cytotoxic
screening for antivirus test were done using Vero cell. Only aqueous and
ethanol extract gives corresponding CC50 value at 6.4±0.3 mg/mL
and 7.9±1.28 mg/mL. Later on, antivirus test using Type 1 Herpes simplex virus
(HSV-1)
infecting Vero cell were conducted with pre and post treatment method. All
extracts showed insignificant activity against HSV-1 in these two
methods. Antibacterial screening was done using a list of gram negative and
positive bacteria using a series of diluted extracts at concentration 10, 5,
2.5, 1.25 and 0.625 mg/mL. All tested extracts did not show any antibacterial
activity since growth inhibition zone were absent in all petri dish. However,
antioxidant test showed high percentage of oxygen radical exclusion’s rate
especially by the ethanol extracts i.e. 93.6%. This was followed by aqueous
(92.65%), methanol (88.83%) and polysaccharide (85.75%) extracts. Viewing the
presence of a significant antioxidative activity with the absence of cytotoxic,
antivirus and antibacterial activities for various Amauroderma sp.
extracts, it can be concluded that these extracts have specific potential as a
natural antioxidative components.
Keywords: Amauroderma sp.; antibacteria; antioxidant; antivirus; cytotoxicity
RUJUKAN
Amakura, Y., Umino, Y., Tsuji, S., Ito, H.,
Hatano, T., Yoshida, T. & Tonogai, Y. 2002. Constituents and their
antioxidative effects in eucalyptus leaf extract used as a natural food
additive. J. Food. Chem 77: 47-56.
Eo, S.K., Kim, Y.S., Lee, C.K. & Han, S.S.
1999. Antiviral activities of various water and methanol metanol soluble
substances isolated from Ganoderma lucidum. Journal of Ethnopharmacology 68:
129-136.
Freshney, R.I. 2006. Culture of Cells for Tissue
Engineering. New York: John Wiley & Sons, Inc.
Kirk, P.M., Cannon, P.F., Minter, D.W. &
Stalpers, J.A. 2008. Dictionary of the Fungi. Edisi ke-10 UK: CAB
International.
Kujumgiev, A., Tsvetkova, I., Serkedijeva, Y.,
Christov, R. & Popov, S. 1999. Antibacterial, antifungal and antiviral
activity of propolis of different geographic origin. Journal of
Ethnopharmacology 64: 235-240.
Lee, S.S., Chang, Y.S. & Noraswati, M.N.R.
2009. Utilization of macrofungi by some indigenous communities for food and medicine
in Peninsular Malaysia. Forest Ecology and Management 257: 2062-2065.
Lee, Y.L., Huang, G.W., Liang, Z.C. & Mau,
J.L. 2007a. Antioxidant properties of three extracts from Pleurotus ccitrinopileatus.
LWT. 40: 823-833.
Lee, Y.L., Yen, M.T. & Mau, J.L. 2007b.
Antioxidant properties of various extracts from Hypsizigus marmoreus. Food Chemistry 104: 1-9.
Lien, E.J., Ren, S., Bui, H.H. & Wang, R.
1999. Quantitative structure: Activity relationship analysis of phenolic
antioxidants. Free Radical Biology & Medicine 26: 285-294.
Lindequist, U., Niedermeyer, T.H.J. &
Julich, W.D. 2005. The pharmacological potential of mushroom. Evid Based
Complement Alternative Medicine 2: 285-299.
Liu, D., Hu, Z., Liu, Z., Yang, B., Tu, W. &
Li, L. 2009. Chemical composition and antimicrobial of essential oil isolated
from the cultured mycelia of Ganoderma japonicum. Journal of Nanjing Medical
University 23:168-172.
Liu, J.J., Huang, T.S., Hsu, M.L., Chen, C.C.,
Lin, W.S., Lu, F.J. & Chang, W.H. 2004a. Antitumor effects of the partially
purified polysaccharides from Antrodia camphorata and the mechanism of
its action. Toxicology and Applied Pharmacology 201: 186-193.
Liu, J., Yang, F., Ye, L.B., Yang, X.J., Timani,
K.A., Zheng, Y. & Wang, Y.H. 2004b. Possible mode of action of antiherpetic
activities of a proteoglycan isolated from the mycelia of Ganoderma lucidum
in vitro. Journal of Ethnopharmacology 95: 265-272.
Mat Rasol, A., Mutaat, H.H., Ito, H. & Kume,
T. 1998. Selection of microorganisms and fermentation condition of oil palm
empty fruit bunch (EFB) and palm press fibre (PPF). Jurnal Sains Nuklear
Malaysia 16: 31-40.
Roberts, L.M. 2004. Australian Ganoderma:
Identification, Growth and Antibacterial Properties. Thesis PhD. Swinburne
University of Technology (unpublished).
Saltarelli, R., Ceccaroli, P., Lotti, M.,
Zambonelli, A., Buffalini, M., Casadei, L., Vallorani, L. & Stocchi, V.
2009. Biochemical characterisation and antioxidant activity of mycelium of Ganoderma
lucidum from Central Italy. Food Chemistry 116: 143-151.
Su, C.T., Hsu, J.T.A., Hsieh, H.P., Lin, P.H.,
Chen, T.C., Kao, C.L., Lee, C.N. & Chang, S.Y. 2008. Anti-HSV activity of
digitoxin and its possible mechanisms. Antiviral Research 79: 62-70.
Tseng, Y.H., Yang, J.W. & Mau, J.L. 2008.
Antioxidant properties of polysaccharides, from Ganoderma tsugae. Food
Chemistry 107: 732-738.
Venzon, M., Rosado, M.C., Fadini, M.A.M.,
Ciociola, A.I. & Pallini, A. 2005. The potential of NeemAzal for the
control of coffee leaf pests. Crop Protection 24: 213-219.
Wang, Y., Ye, L. & Leung, L.K. 2008. A
positive feedback pathway of estrogen biosynthesis in breast cancer cells is
contained by resveratrol. Toxicology 248: 130-135.
Wasser, S.P. 2002. Medicinal mushrooms as a
source of antitumor and immunomodulating polysaccharides. Appl Microbiol.
Biotechnol. 60: 258-274.
Zhao,
L., Dong, Y., Chen, G. & Hua, Q. 2010. Extraction, purification,
characterization and antitumor activity of polysaccharides from Ganoderma
lucidum. Carbohydrate Polymers 80: 783-789.
*Pengarang untuk surat-menyurat; email: eismail@ukm.my
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