Sains Malaysiana 42(9)(2013):
1253–1259
Antioxidant
Activity in Crude Petroleum Benzene, Chloroform, Methanol
and Water Extracts of
Six Selected Vegetables
(Aktiviti Antioksida dalam Ekstrak Mentah Petroleum Benzena,
Kloroform, Metanol dan Air daripada Enam Sayuran Terpilih)
Sumathi Murti1*, Nurhayati Zainal Abidin1 & Ashril Yusof2
1Institute of Biological Science, Faculty of Science, University
of Malaya
50603 Kuala Lumpur, Malaysia
2Sport Centre, University of Malaya, 50603 Kuala Lumpur, Malaysia
Diserahkan: 2 Ogos 2012 /Diterima: 2 Februari 2013
ABSTRACT
In this study, crude petroleum benzene, chloroform, methanol and
water extracts of six selected vegetables namely, garlic chives (Allium tuberosum), celery (Apium
graveolens (L.)), sweet potato leaves (Ipomoea batatas (L.)), curry
leaves (Murraya koenigii (L.)), winged beans (Psophocarpus
tetragonolobus) and sweet leaves (Sauropus androgynus) were tested
for antioxidant activities using three bioassays namely 2,
2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, reducing power
assay and metal chelating assay. Overall, among the 24 crude extracts tested,
petroleum benzene and chloroform extract of Murraya koenigii (L.) showed
higher free radical scavenging activities (IC50 = 0.02 mg/mL and 0.0225
mg/mL, respectively) when compared to ascorbic acid (IC50 = 0.00375 mg/mL) and
strong reducing powers with absorbance value of 1.208 ± 0.006 and 1.833 ± 0.003
when compared to butylated hydroxyanisole with absorbance value of 2.625 ±
0.004 at the highest concentration tested, 1 mg/mL in both DPPH and reducing power
assay, respectively. In metal chelating assay, methanol, petroleum benzene and
chloroform extracts of Murraya koenigii (L.) showed moderate metal
chelating activities of 88.60 ± 0.02%, 78.30 ± 0.23% and 73.61 ± 0.22%,
respectively, at 1 mg/mL when compared to ethylenediaminetetraacetic acid which
is 98.63 ± 0.13%. These findings suggested the important significance of Murraya
koenigii (L.) consumption in prevention of diseases.
Keywords: Curry leaves; DPPH radical scavenging assay; metal chelating
assay; reducing power assay
ABSTRAK
Dalam kajian ini, ekstrak mentah petroleum benzena, kloroform,
metanol dan air daripada enam sayur-sayuran terpilih iaitu, daun kucai bawang
putih (Allium tuberosum),
saderi (Apium graveolens (L.)), daun ubi keledek (Ipomoea batatas (L.)),
daun kari (Murraya koenigii (L.)), kacang botol (Psophocarpus
tetragonolobus) dan daun manis (Sauropus androgynus) telah diuji
untuk aktiviti antioksidan menggunakan tiga bioasai iaitu asai penyahbebas
radikal 2, 2-difenil-1-picrylhydrazyl (DPPH), asai kuasa penurun dan asai pengkelat
logam. Secara keseluruhannya, antara 24 ekstrak mentah yang diuji, ekstrak
petroleum benzena dan kloroform Murraya koenigii (L.) menunjukkan
aktiviti penyahbebas radikal yang tinggi (IC50 = 0.02 mg/mL dan 0.0225
mg/mL masing-masing) berbanding dengan asid askorbik (IC50 = 0.00375 mg/mL) dan
kuasa penurun yang kuat dengan nilai keserapan 1.208 ± 0.006 dan 1.833 ± 0.003
berbanding dengan hydroxyanisole butylated dengan nilai keserapan 2.625 ± 0.004
pada kepekatan tertinggi yang diuji, 1 mg/mL pada kedua-dua asai DPPH dan
kuasa penurun, masing-masing. Dalam asai pengkelat logam, ekstrak metanol,
petroleum benzena dan kloroform Murraya koenigii (L.) menunjukkan
aktiviti pengkelat logam yang sederhana 88.60 ± 0.02%, 78.30 ± 0.23% dan 73.61
± 0.22% masing-masing pada 1 mg/mL berbanding dengan asid
atelindiamintetrasetik (98.63 ± 0.13%). Penemuan ini menunjukkan kepentingan
penggunaan Murraya koenigii (L.) dalam pencegahan penyakit.
Kata kunci: Asai kuasa
penurun; asai pengkelat logam; asai penyahbebas radikal DPPH;
daun kari
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*Pengarang
untuk surat-menyurat; email: m_sumi2001@yahoo.com
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