Sains Malaysiana 41(9)(2012): 1071–1077

 

 

Optimization of Protein Content in Earthworm-based Fish Feed Formulation

for Catfish (Clarius gariepinus)

Pengoptimuman Kandungan Protein di dalam Formulasi Makanan Ikan-Berasaskan

Cacing Tanah untuk Ikan Keli (Clarius gariepinus)

 

Zarina Zakaria*, Noor Hasyierah Mohd Salleh, Alina Rahayu Mohamed, N.G. Ahmad Anas

& Siti Nur Amirah Idris

School of Bioprocess Engineering, Universiti Malaysia Perlis, 02600 Arau, Perlis, Malaysia

 

Diserahkan: 13 September 2011 / Diterima: 25 April 2012

 

 

ABSTRACT

The optimization of protein content in earthworm-based fish feed formulation was investigated in the study. Full factorial design (FFD) and central composite design (CCD) were used as a statistical tool to screen the range of the selected parameters and to optimize the process, respectively. Three components were combined in the fish feed formulation namely earthworm powder, chicken guts and soybean waste as the protein source. The optimum nutritional values were obtained at 25%, 25% and 5.95% for earthworm powder, soybean waste and chicken guts, respectively, to give an optimum protein content of 35.97%. Formulation of fish feed from earthworm powder can be an alternative in aquaculture industry in the near future.

 

Keywords: Catfish; earth worm powder; fish feed formulation; optimization

 

ABSTRAK

Pengoptimuman kandungan protein dalam formulasi makanan ikan berasaskan cacing tanah dikaji. Reka bentuk Faktorial Penuh dan Reka bentuk Komposit Berpusat telah digunakan untuk analisis dalam kajian ini bagi mengenal pasti julat parameter terpilih serta mengoptimum proses masing-masing. Tiga komponen telah digabungkan dalam formulasi makanan ikan iaitu tepung cacing tanah, usus ayam dan sisa kacang soya sebagai sumber protein. Nilai nutrisi optimum yang diperoleh adalah masing-masing 25%, 25% dan 5.95% bagi tepung cacing tanah, sisa kacang soya dan usus ayam yang memberikan kandungan protein optimum 35.97%. Formulasi makanan ikan dengan tepung cacing tanah boleh menjadi alternatif dalam industri akuakultur di masa hadapan.

 

Kata kunci: Formulasi makanan ikan; ikan keli; pengoptimuman; tepung cacing tanah

 

RUJUKAN

 

Aksnes, A., Izquierdo, M.S., Robaina, L., Vergara, J.M. & Montero, D. 1997. Influence of fish meal quality and feed pellet on growth, feed efficiency and muscle composition in gilthead seabream (Sparusaurata). Aquaculture 153: 251-261.

Barrows, F.T., Gaylord, T.G., Sealey, W.M., Porter, L. & Smith, C.E. 2008. The effect of vitamin premix in extruded plant-based and fish meal based diets on growth efficiency and health of rainbow trout (Oncorhynchusmykiss). Aquaculture 283:148-155.

Bransden, M.P., Carter, C.G. & Nicholos, P.D. 2003. Replacement of fish oil with sunflower oil in feeds for Atlantic salmon (Salmosalar L): effect on growth performance, tissue fatty acid composition and disease resistance. Comparative Biochemistry and Physiology 135: 611-625.

Carter, C.G. & Hauler, R.C. 2000. Fish meal replacement by plant meals extruded feeds for Atlantic salmon (Salmosalar L). Aquaculture 185: 299-311.

Cavalheiro, J.M.O., Souza, E.O. & Bora, P.S. 2007. Utilization of shrimp industry waste in the formulation of tilapia (Oreochromis niloticus Linnaeus) feed. Bioresource Technology 98: 602-606.

Info Fish International. 2010. Tropical freshwater fish-Asia’s fast track aquaculture product: 49-57.

Ishii, Y. & Mihara, H. 1992. Process For The Production of Dried Earthworm Powder and Antihyperlipemic, Antidiabetic, Antihypertensive and Antihypotensive Preparations Containing Dried Earthworm Powder as Active Ingredients. US Patent 5128148.

Kenneth, R.T., Laura, A.M., Linda, S.E. & Carl, D.W. 2005. Evaluation of practical diets containing different protein levels, with or without fish meal, for juvenile Australian red claw crayfish. Aquaculture 244: 241-249.

Khan, M.S. 1994. Apparent digestibility coefficients for common feed ingredients in formulated diets for tropical catfish, Mystusnemurus (Cuvier & valenciennes). Aquaculture Research 25: 167-174.

Llenart, M., Handlinger & J. Powell, M. 2003. Treatment of spionid mud worm (Boccardiaknoxi Rainer) infestation of cultured abalone. Aquaculture 217: 1-10.

Medina A.L., Cova, J.A., Vielma, R.A., Pujic, P., Carlos, M.P. & Torres, J.A. 2003. Immunological and chemical analysis of proteins from Eiseniafoetida earthworm. Food and Agricultural Immunology 15 (3-4): 25-263.

Mukherjee, A.K., Kalita, P., Unnj, B.G., Wann, S.B., Sajkia, D. & Mukhopadhyay, P.K. 2010. Fatty acid composition of four potential aquatic weeds and their possible use as fish feed neutraceuticals. Food Chemistry 123: 1252-1254.

Myers, R.H. & Montgomery, D.C. 2002. Response surface methodology: Process and product optimization using designed experiments. 2nd ed. New York: John Wiley & Sons.

Nhi, N.H.Y. 2000. Utilization of earthworms (Perionyx excavatus) as a protein source for growing fingerling marble goby (Oxyeleotris marmoratus) and tra catfish (Pangasiushy pophthalmus). Thesis, Department of Animal Nutrition and Management, Swedish University of Agricultural Science (unpublished)

Paoletti, M.G., Buscardo, E., VanderJagt, D.J., Pastuszyn, A., Huang, Y.S., Chuang, L.T, Millson, M., Cerda, H., Torees, F & Glew, R.H. 2003. Nutrient contents of earthworms consumed by Ye’Kuana Amerindians of the Alto Orinoco of Venezuela. Proceeding Biological Sciences 1512: 247-257.

Refstie, S., Skjaervik, O.F. & Rosenlund, G. 2006. Feed intake, growth and utilization of macronutrients and amino acids by 1- and 2- years old Atlantic cod (Gadusmorhua) fed standard or bioprocessed soybean meal. Aquaculture 255: 279-291.

Robinson, H.E. & Li, H.M. 2007. Catfish Protein Nutrition: Revised. Mississippi Agricultural & Forestry Experiment Station Bulletin April.

Ruohonen, K. & Kettunen, J. 2004. Effective experimental designs for optimizing fish feeds. Aquaculture Nutrition 10: 145-151.

Saalah, S., Shapawi, R., Othman, N.A. & Bono, A. 2009. Effect of formula variation in the properties of fish feed pellet. In Proceeding of 3rd International Conference on Chemical & Bioprocess Engineering, 12th-14th August 2009, University Malaysia Sabah, Kota Kinabalu, Malaysia.

Sinha, R.K., Heart, S. & Valani, D. 2010. Earthworms-the environmental engineers: a review of vermiculture technologies for environmental management and resource development. International Journal of Global Environmental Issues 10: 265-292.

Tacon, A.G.J. 1990. Fish feed formulation and production. Report for Fisheries Development in Qinghai Province. November 1990.

Xu, Z., Lin, X., Yang, Y. & Wang, Y. 2007. Nitrogen, phosphorus and energy waste output of four marine cage-cultured fish fed with trash fish. Aquaculture 263: 130-141.

 

*Pengarang surat-menyurat; email: zarinaz@unimap.edu.my

 

 

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