Sains Malaysiana 40(9)(2011): 959–964
Pengecaman Awal Komuniti Bakteria Sel Bahan Api Mikrob
dalam Air Sisa Kumbahan
(Preliminary
Identification of the Microbial Fuel Cell Bacteria Communities in Sewage)
S.M. Zain*, R. Hashim, N.S. Roslani, F. Suja & N.E.A. Basri,
S.M. Zain*,
Department of
Civil & Structural Engineering, Faculty of Engineering & Built
Environment
Universiti
Kebangsaan Malaysia, 43600 Bangi, Selangor D.E., Malaysia
N. Anuar &
W.R.W. Daud
Department of
Chemical Process & Engineering, Faculty of Engineering & Built
Environment
Universiti
Kebangsaan Malaysia, 43600 Bangi, Selangor D.E., Malaysia
Diserahkan: 16
Jun 2010 / Diterima: 11 Januari 2011
ABSTRAK
Kajian ini bertujuan untuk
menentukan jenis bakteria yang hadir di dalam air sisa kumbahan yang dapat
membantu menghasilkan tenaga elektrik dan pada masa yang sama dapat
menyingkirkan kandungan karbon dan nitrogen. Kaedah pengesanan kumpulan
bakteria yang digunakan adalah teknik penghibridan in situ berpendarfluor (FISH)
manakala kaedah tindak balas berantai polimerase (PCR)
untuk mengenalpasti bakteria tersebut. Penentuan ciri biokimia menggunakan BIOLOG
GEN III MICROPLATE™ turut digunakan. Bakteria
yang didapati daripada air sisa dikultur dan ditulenkan di atas agar zat
makanan untuk menentukan ciri-ciri morfologi koloni bakteria tersebut.
Berdasarkan pencirian koloni dan pewarnaan Gram, sebanyak 21 pencilan telah
diperolehi daripada tiga lokasi sampel air sisa kumbahan daripada loji rawatan
enap cemar teraktif (enam koloni air sisa mentah; sebelas koloni tangki
pengudaraan; empat koloni tangki kitaran enap cemar teraktif). Penentuan awal
daripada ketiga-tiga kaedah yang dijalankan tidak dapat memberikan padanan yang
tepat dengan hanya mencatatkan kehadiran bakteria pengoksida ammonia (FISH)
dan Kurthia Gibsoni (BIOLOG) di dalam sampel tangki
pengudaraan; bakteria Bacillus sp (PCR) dan Bacillus
Pseudomycoides (BIOLOG) di dalam sampel kitaran enap
cemar teraktif. Ketumpatan kuasa maksimum yang diperoleh daripada sampel
kitaran enap cemar teraktif adalah 9.053 mW/cm2 dengan
tahap penyingkiran COD dan jumlah nitrogen adalah (TKN)
masing-masing 26.8% dan 40%.
Kata kunci: Air sisa
kumbahan; bakteria; penghibridan in situ berpendarfluor (FISH);
tindak balas berantai polimerase (PCR); sel bahan api mikrob
ABSTRACT
This study aimed to
determine the types of bacteria exist in wastewater that contibute to generate
electricity and simultaneously remove carbon and nitrogen. The method used was
Fluorescence In Situ Hibridization (FISH) to detect the bacteria
group while Polymerase Chain Reaction (PCR) was used to confirm the
observation made using FISH. A biochemical identification
using BIOLOG GEN III MICROPLATE™ also was used . The
samples were cultured on nutrient agar plate to identify the morphology of the
bacteria. The result showed that 21 isolates from three different locations at
the activated sludge treatment plant with six, eleven and four strains for raw
sewage, aeration tank and returned activated sludge samples, respectively.
Preliminary identification does not give a good match but only showed the
existence of ammonia-oxidizing bacteria (FISH) and Kurtia Gibsoni (BIOLOG)
from aeration tank : Bacillus sp (PCR) and Bacillus
Pseudomycoides (BIOLOG) from returned activated
sludge. The maximum power density generated using returned activated sludge was
9.053 mW/cm2, with 26.8% COD removal and 40% TKN removal.
Keywords: Bacteria; Fluorescence In Situ Hibridization(FISH);
microbial fuel cell; polymerasa chain reaction(PCR)
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*Pengarang untuk
surat-menyurat; email: smz@eng.ukm.my
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