Sains Malaysiana 40(10)(2011): 1147–1151

Pattern of Crevicular Alkaline Phosphatase During Orthodontic

Tooth Movement: Leveling and Alignment Stage

(Corak Alkalin Fosfatase Krevikel Semasa Pergerakan Gigi Ortodontik: Peringkat Penyusunan Gigi)

Asma Alhusna Abang Abdullah* & Rohaya Megat Abdul Wahab

Department of Orthodontic, Faculty of Dentistry, Universiti Kebangsaan Malaysia

Jln Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia

Shahrul Hisham Zainal Ariffin

Faculty of Science and Technology, Universiti Kebangsaan Malaysia

43600 UKM Bangi, Selangor, Malaysia

Diserahkan: 15 Julai 2010 / Diterima: 21 Oktober 2010

ABSTRACT

Bone formation has been associated with the presence of an enzyme called alkaline phosphatase (ALP). This longitudinal study was carried out to observe its activity in gingival crevicular fluid (GCF) during leveling and alignment stage of orthodontic fixed appliance treatment. Fourteen patients between the ages of 15 and 27 years old with moderate upper labial segment crowding were recruited from postgraduate orthodontic clinic. GCF from mesial and distal sites of upper canines were collected using endodontic paper point before the fixed appliance placement (week 0). The baseline level of ALP (week 0) acted as a control. Samplings of GCF were repeated at 1st, 2nd and 3rd week during orthodontic treatment. The activities of ALP were measured using spectrophotometer (405 nm). Paired sample t-test was used to assess the significance of difference over the 3 weeks. Although the results showed patterns of ALP activities on the test teeth throughout the 3 weeks of leveling and alignment stage, however the results were not significant (p >0.05) when compared to control. Therefore, it has been shown that there was no significant pattern of ALP activities in GCF in patients undergoing leveling and alignment stage of orthodontic treatment.

Keywords: Alkaline phosphatase; bone formation; orthodontic

ABSTRAK

Pembentukan tulang telah dikaitkan dengan kewujudan sejenis enzim yang dikenali sebagai akalin fosfatase (ALP). Kajian longitud ini dijalankan untuk memerhati aktiviti enzim tersebut di dalam cecair krevikel gingival (GCF) semasa peringkat penyusunan gigi rawatan aplian tetap ortodontik. Empat belas pesakit berumur di antara 15 dan 27 tahun yang mempunyai kesesakan sederhana di segmen labial atas telah diambil dari klinik pascasiswazah ortodontik. GCF dari kawasan mesial dan distal gigi kanin atas telah diambil menggunakan kertas poin endodontik sebelum peletakkan aplian tetap (minggu 0). Tahap aktiviti ALP pada minggu 0 ini bertindak sebagai kawalan. Pensampelan GCF telah diambil pada minggu pertama,kedua dan ketiga sepanjang rawatan ortodontik dijalankan. Aktiviti ALP diukur menggunakan spektrofotometer (405 nm).Ujian-t sampel berpasang digunakan untuk menilai perbezaan signifikan di antara 3 minggu tersebut. Walaupun hasil kajian mendapati bahawa terdapat corak pada aktiviti ALP sepanjang 3 minggu kajian dijalankan namun ia tidak signifikan (p>0.05) apabila dibandingkan dengan kawalan. Oleh itu, kajian ini telah membuktikan bahawa tiada corak yang signifikan berlaku pada aktiviti ALP di dalam GCF pada pesakit yang menjalankan rawatan ortodontik semasa peringkat penyusunan gigi.

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Kata kunci: Alkalin fosfatase; ortodontik; pembentukan tulang

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*Pengarang untuk surat-menyurat; email: asmaabdullah@yahoo.com