Sains Malaysiana 38(2): 191-196(2009)

Solvent Fermentation From Palm Oil Mill Effluent Using

Clostridium acetobutylicum In Oscillatory Flow Bioreactor

(Penghasilan Pelarut dari Fermentasi Efluen Loji Minyak Sawit oleh

Klostridium Asetobutilikum di Dalam Bioreaktor Aliran Berayun)

M.S. Takriff, N. Masngut, A. A. H. Kadhum,

M. S. Kalil And A. W. Mohammad

Department of Chemical & Process Engineering

Faculty of Engineering,Universiti Kebangsaan Malaysia

43600 Bangi. Selangor Darul Ehsan, Malaysia

Received:  4 January 2008/Accepted:  11 Ogos 2008

ABSTRACT

Acetone-butanol-ethanol (ABE) fermentation from Palm Oil Mill Effluent (POME) by C. acetobutylicum NCIMB 13357 in an oscillatory flow bioreactor was investigated. Experimental works were conducted in a U-shaped stainless steel oscillatory flow bioreactor at oscillation frequency between 0.45-0.78 Hz and a constant amplitude of 12.5 mm. Fermentations were carried out for 72 hr at 35^oC using palm oil mill effluent and reinforced clostridia medium as a growth medium in batch culture. Result of this investigation showed that POME is a viable media for ABE fermentation and oscillatory flow bioreactor has an excellent potential as an alternative fermentation device.

Keywords: Anaerobic fermentation; Clostridium acetobutylicum; oscillatory flow bioreactor; palm Oil Mill Effluent; solvent  

ABSTRAK

Kajian ini fokus kepada penghasilan pelarut asiton, butanol dan etanol (ABE) daripada fermentasi efluen loji minyak sawit oleh C. Acetobutylicum di dalam bioreaktor aliran berayun. Ujikaji makmal telah dijalankan di dalam sebuah bioreaktor aliran berayun berbentuk U yang diperbuat dari keluli tahan karat. Kajian ini dilkasanakan pada julat frekuensi 0.45-0.78Hz dan amplitud malar 12.5 mm. Proses fermentasi dilaksanakan secara sesekumpul selama 72 jam pada suhu 35^oC dengan menggunakan efluen loji minyak sawit (POME) dan media klostridia yang telah diperkukuhkan (RCM) sebagai medium tumbesaran. Keputusan kajian ini menunjukkan POME adalah media tumbesaran yang sesuai bagi penghasilan pelarut oleh C. Aketobutilikum dan bioreaktor aliran berayun menunjukan potensi yang sangat baik sebagai alat fermentasi alternatif.

Kata kunci: Bioreaktor aliran berayun; efluen loji minyak sawit; fermentasi anerobik; Kostridium asetobutulicum; pelarut

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