Sains Ma1aysiana 27: 9-17 (1998) Sains Hayat/
Life Sciences
Mutu Mikrobiologi Susu Lembu Mentah Kawasan
Selatan Semenanjung Malaysia
(Microbiological quality of raw milk from the southern
region of Peninsular Malaysia)
Chye Fook Yee, Aminah Abdullah & Mohd Khan Ayub
Jabatan Sains Makanan dan Pemakanan,
Fakulti Sains Hayat
Universiti Kebangsaan Malaysia,
43600 UKM Bangi Se1angor D.E.
ABSTRAK
Kualiti mikrobiologi susu lembu mentah di kawasan Selatan Semenanjung Malaysia telah dianalisis untuk menentukan status mikrobiologi dan kehadiran patogen terpilih dalam susu tempatan. Susu yang diambil dari 127 ladang ternakan lembu mempunyai min kiraan plat total, koliform dan Escherichia coli yang tinggi, iaitu masing-masing 5.5 ´ 105 - 2.7 ´ 107/ml, 2.4 ´ 104-8.0 ´ 105/ml dan 3.5 ´ 103 - 4.6 ´ 104/ml. Hampir 63% daripada sampel susu dicemari oleh Staphylococcus aureus manakala 33% adalah positif bagi E. coli 0157:H7. Sebanyak 5 serotip salmonella termasuk S. agona, S. newport, S. heldelberg, S. typhimurium dan S. weltevreden telah dikenal pasti. Sementara itu, sebanyak 18 strain Listeria spp. telah dipencilkan daripada 4.2% sampel yang positif.
ABSTRACT
The microbiological quality of raw milk from the southern region of Peninsular Malaysia was analyzed for the microbial status as well as the prevalence of selected emerging pathogens in local milk. The mean counts per ml for total plate, coliform and Escherichia coli were generally high for all samples collected from 127 dairy farms, with a range of 5.5 ´ 105 - 2.7 ´ 107/ml, 2.4 ´ 104-8.0 ´ 105/ml dan 3.5 x 103 - 4.6 x 104/ml respectively. Approximately 63% of milk samples were contaminated with Staphylococcus aureus and 33% were E. coli 0157:H7 positive. Five serotypes of Salmonella were identified including S. agona, S. newport, S. heldelberg, S. typhimurium and S. weltevreden. Meanwhile 18 strains of Listeria spp. were isolated from 4.2% of the positive samples.
RUJUKAN/REFERENCES
APHA. 1992. Compendium of methods for the microbiological examination of Foods. American Public Health Association, Washington. 325-367.
Bames-Pallesen, F.D., Blackmer, P. Britten, A., Bushnell, R.B., Demme, V, Welcome, F. 1987. Laboratory and field handbook on bovine mastitis. Natl. Matitis Counc. Arlington, VA.
Bergdoll, M.S. 1989. Staphylococcus aureus. Dlm. Doyle, M.P. (pnyt). Foodbome bacterial pathogens. Markel Dekker Inc. New York. 463-523.
Burgess, K., Heggum, C., Walker, S. & Van Schothorst, M. 1993. Recommendations for the hygienic manufacture of milk and milk products: Spoilage and pathogenic bacteria in milk based products. International Dairy Federation Bull. 292:28-32.
Burt, R & Wellsteed, S. 1991. Food safety and legislation in dairy industry. J. Soc. of Dairy Technol. 44(3): 80-82.
Dominguez Rodriquez, L., Fernandez Garayzabal, J.F., Valquez Boland, J.A, Rodriquez Ferri, E & Suarez Fernandez, G. 1985. Isolation de microorganisms de genre Listeria a partir de lait cru destine a la consomnation humaine. J. of Microbiol. 31: 938-941 (Abstract).
Eberhart, R.J. & Buckalew, J.M. 1972. Intramammary infections in a dairy herds with a low incidence of Streptococcus agalactiae and Staphylococcus aureus. J. Am. Vet. Med. Assoc. 171: 630-634.
Farber, J.M., Sanders, G.W. & Malcolm, S.A. 1988. The presence of Listeria spp. n raw milk in Ontario. Canadian J. Microbiol. 34: 95-100.
Feresu, S. & Nyati, H. 1990. Fate of pathogenic and non-pathogenic Escherichia coli strains in two fermented milk products. J. Appl. Bacteriol. 69: 814-821.
Gonzales, R.N., Jasper, D.E., Kronlund, N.C., Farver, T.B., Cullor, J.S., Bushnell, R.B. & Delinger, J.D. 1990. Clinical mastitis in two California dairy herds participating in contagious mastitis control programme. J. Dairy Sci. 73: 648.
Griffin, P.M. & Tauxe, RV. 1991. The epidemiology of infection caused by Escherichia coli 0157:H7, other enterohaemorrhagic E. coli and the associated hemolytic uremic syndrome. Epidemiol. Rev. 13: 60-98.
Harmon, R.J. & Lenglois, B.E. 1989. Mastitis due to coagulase negative Staphyococcus species. Agri. Pract. 10: 29-32.
Harvey, J. & Gilmour., A 1992. Occurrence of Listeria species in raw milk and dairy products in Northern Ireland. J. Appl. Bacteriol. 72: 119-125.
Hayes, P.S., Feeley, J.C., Graves, L.M., Ajello, G.W. & Fleming D.W. 1986. Isolation of Listeria monocytogenes from raw milk. Appl. Environm. Microbiol. 51: 438-440.
Holmberg, S.D., Wells, J.G. & Cohan, M.L. 1984. Animal-to-man transmission of antimicrobial resistant Salmonella: investigation of US outbreaks. 1971-1983. Science. 225: 833.
Humphrey, T.J. & Hart, R.J.C. 1988. Campylobacter and Salmonella contamination of unpasteurised cows milk on sale to the public. J. Appl. Bacteriol. 65: 463-467.
ISO. 1990. Microbiology - General guidance on method for the detection of Salmonella. International standards Organisation. Geneva. ISO 6579.
Lund, AM., Zottola, E.A. & Pusch, D.J. 1991. Comparison of methods for isolation of Listeria from raw milk. J. Food Proto 54: 602-605.
March, S.B. & Ratnam, S. 1986. Sorbitol MacConkey medium for the detection of Escherichia coli 0157:H7 associated with hemorrhagic colitis. J. Clin. Microbiol. 23: 869-872.
Marshall, RT. 1993. Standard methods for examination of dairy products. 16th ed. American Public Health Association. Washington.
Martin, L.M., Shipman, L.D., Wells, J.G., Wachsmuth, 1.K., Tauxe, R.V. Davies, J.P., Arnoldi, J. & Tilleli, J. 1986. Isolation of Escherichia coli 0157:H7 from dairy cattle associated with two cases of hemolytic uraemic syndrome. Lancet. 2: 1043-1045.
McEwen, S.A, Martin.. S.W., Clarke, RC. & Tamblyn, S.B. 1988. A prevalence survey of Salmonella in raw milk in Ontario. J. Food Proto 59: 963-965.
Montenegro, M.A, Bulte, M., Trumpf, T. A1ksic, S., Reuter, G., Bulling, E. & Helmuth, R. 1990. Detection and characterisation of feacal verotoxin-producing Escherichia coli from healthy cattle. J. Clin. Microbiol. 28: 1417-1421.
Padhye, V.V. & Doyle, M.P. 1992. Escherichia coli 0157. H7: Epidemiology, pathogenesis and methods for detection in foods. J. Food Proto 55: 555-556.
Potter, M.E., Kaufmann, AF., Blake, P.A & Feldman, R.A 1984. Unpasteurised milk. The hazard of health fetish. J. Am. Med. Assoc. 252: 2048.
Vamam, A.H. & Evans, M.G. 1991. Foodborne pathogens. An illustrated text. Wolf Pub. Ltd. England.
Vasavada, P.C. 1988. Pathogenic bacteria in milk - A review. J. Dairy Sci. 71: 2809-2816.
Westoo, A & Peterz, M. 1992. Evaluation of methods for the detection of Listeria monocytogenes in foods: NMKL collaborative study. J. AOAC. Int. 75: 46-52.
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