Sains Ma1aysiana 25(1): 99-109 (1996) Sains Hayat/ Life Sciences
Prenatal Caffeine Exposure Modifies Development and Reflexes of Neonates and Anxiety of Young Adult Mice
Jamaan S. Ajarem & Mohammad Ahmad Dept of Zoology, King Saudi University P.O. Box 2455 Riyadh 11451 Saudi Arabia
ABSTRACT
The effects of prenatal caffeine exposure were examined on the morphological development and early development of reflexes in the mice pups, and further, the anxiety was also studied in the weaned mice using the plus-maze test. It was found that the postnatal body weight gain of the treated pups declined significantly. The normal eye-opening and hair appearance were also affected in the pups due to caffeine treatment. Measurement of early development of sensory motor reflexes in the pups showed that during the first week, caffeine had significantly stimulated the righting reflex, cliff avoidance and rotating reflexes with interaction between age and treatment doses. It was found in the plus-maze test that caffeine had significantly reduced the percentages of time spent as well as the entries into the open arms indicating for an anxiogenic action of caffeine in the young adult mouse. Also, caffeine stimulated the locomotor activity in the mouse as exemplified by an increase in the total number of arm entries in the plusmaze task. The present data support the basic conclusion that prenatal caffeine has a direct in utero action on the reflexes of the developing mouse pups and the anxiogenic action produced in them is longer lasting in nature.
RUJUKAN/REFERENCES
Aeschbacher, H.V., Milon, H., Poot, A. & Wurzner, H.P. 1980. Effect of caffeine on rat offspring from treated dams. Toxicol. Lett. 7: 71-77. Ajarem, J.S. 1985. Lasting behavioural consequences of perinatal exposure of laboratory mice to a variety of drugs. Ph. D. thesis, University of Wales. Ajarem, J.S. & Ahmad, M. 1991. Behavioral and biochemical consequences of perinatal exposure of mice to instant coffee: a correlative evaluation. Pharmacol. Biochem. Behav. 40: 847-852. Ajarem, J.S. & Brain, P.F. 1993. Prenatal caffeine exposure modifies behavioural responses in mice. Behav. Pharmacol. 4: 541-544. Charney, D.S., Galloway, M.P. & Heninger, G.R. 1984. The effects of caffeine on plasma MHPG, subjective anxiety, autonomic symptoms and blood pressure in healthy humans. Life Sci. 35: 135-144. Concannon, J.T. Braughler, J.M. & Schechter, M.D. 1983. Pre- and postnatal effects of caffeine on brain biogenic amines, cyclic nucleotides and behaviour in developing rats. J. Pharmacol. Exptl. Therapeut. 226: 673-679. Dunlop, M. & Court, J.M. 1981. Effect of maternal caffeine ingestion on neonatal growth in rats. Bioi. Neonate, 39: 178-184. Fabro, S. & Sieber, S.M 1969. Caffeine and nicotine penetrates the pre-implantation blastocyte. Nature (Lond) 223: 410-411. Fujii, T. & Nishimura, H. 1972. Adverse effects of prolonged administration of caffeine on rat fetus. Toxicol. Appl. Pharmacol. 22: 449-457. Gilbert, E.F. & Pisty, W.R. 1973. Effect on the offspring of repeated caffeine administration to pregnant rats. J. Reprod. Fert. 34: 495-499. Goldstein, A. & Warren, R. 1962. Passage of caffeine into human and rodent gonadal tissue. Biochem. Pharmacol. 11: 166-168. Golli, C., Spano, P.F. & Szyska, K. 1975. Accumulation of caffeine and its metabolites in rat fetal brain and liver. Pharmacol. Res. Commun. 7: 217-221. Holloway, W.R. Jr. 1982. Caffeine: Effects of acute and chronic exposure on the behaviour of neonatal rats. Neurobehav. Toxicol. Teratol. 4: 21-32. Kaplan, G.B., Tai, N.T. Greenblatt, D.J. & Shader, R.I. 1990. Separate and combined effects of caffeine and alprazolarn on motor activity and benzodiazepine receptor binding in vivo. Psychopharmacology 101: 539-544. Lister, R.G. 1987. The use of a plus-maze to measure anxiety in the mouse. Psychopharmacology 92: 180-185. Nehlig, A., Daval, J. & Debry, G. 1992. Caffeine and the central nervous system: mechanisms of action, biochemical, metabolic and psychostimulant effects. Br. Res. Rev. 17: 139-170. Palm, P.E., Arnold, E.p., Rachwall, P.C., Leyczek, J.C., Teague, K.W. & Kensler, C.J. 1978. Evaluation of the teratogenic potential of fresh-brewed coffee and caffeine in the rat. Toxicol. Appl. Pharmacol. 44: 1-6. Pellow, S., Chopin, P., File, S.E. & Briley, M. 1985. Validation of closed: open an entries in an elevated plus-maze as a measure of anxiety in the rat. J. Neurosci. Methods 14: 149-167. Pollard, I., Jabbour, H. & Meharabani, P.A. 1987. Effects of caffeine administered during pregnancy on fetal development and subsequent function in the adult rat: prolonged effects on a second generation. J. Toxicol. Environ. Health 22: 1-15. Sinton, C.M., Valatx, J.L. & Jouvet, M. 1981. Gestational caffeine modifies offspring behaviour in mice. Psychopharmacology, 75: 69-74. Sokal, R.R. & Rohlfe, F.J. 1981. Biometry: The Principles and practice of statistics in biological research. San Francisco: W.H. Freeman. Spindel, E., Arnold, M., Cusak, B. & Wurtman, R.J. 1980. Effects of caffeine on anterior pituitary and thyroid hormones in the rat. J. Pharmacol. Exptl. Ther. 214: 58-62. Tanaka, H., Nakazawa, K., Arimur, M. & Iwaski, S. 1984. Caffeine and its dimethylxanthines and fetal cerebral development in rat. Br. Developt. 6: 355-361. Thayer, P.S. & Kensler, C.J. 1973. Exposure of four generations of mice to caffeine in drinking water. Toxicol. Appl. Pharmacol. 25: 169-179. Uhde, T.W., Boulenger, J.P., Jimmerson, D.C. & Post, R.M. 1984. Caffeine: relationship to human anxiety, plasma MHPG and cortisol. Psychopharmacol. Bull. 20: 426-430. West, G.L., Sobotka, T.J., Brodie, R.E., Beier, J.M. & O'Donnell, M.W. Jr. 1986. Postnatal neurobehavioral development in rats exposed in utero to caffeine. Neurobehav. Toxicol. Teratol. 8: 29-43. Yamane, T. 1973. The t- Distribution. In: Statistics, an introductory analysis. Yamane, T. ed., 3rd. Edn., London: Harper and Row Publishers, pp. 647-650.
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